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Figure 3. Metabolic activity of insulin glargine and its metabolites in rat adipocytes.

To compare the metabolic activity of glargine and its metabolites insulin-stimulated lipid synthesis in isolated primary rat adipocytes was analyzed by incorporation of [3- 3 H]glucose into toluene-extractable lipids and subsequent measurement of radioactivity by liquid scintillation counting. The adipocytes were treated with increasing concentrations of insulin, analogs, metabolites or IGF-1 for 90 min at 37°C in the presence of [3- 3 H]glucose. Each point represents the mean ± SEM of five different adipocyte preparations with activity measurements done in duplicate.

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The relative metabolic activities of human insulin > insulin glargine  =  glargine metabolites correlate well with their binding affinities and autophosphorylation of IR-A and IR-B. This is compatible with previous studies demonstrating that ligand affinity and activation (i.e. autophosphorylation) of the IR are the key events for initiation of downstream signaling to the metabolic effector systems of insulin and its analogs Felmini GIANINA Ankle boots targof/metropolis black TyZJLc
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The maintenance of binding, autophosphorylation and metabolic activities of insulin analogs with modified carboxy-terminus confirm the previous findings that amino acid modifications at the insulin B-chain beyond position B25, in particular the B26-B30 region, do not play a critical role in its recognition by both isoforms of the IR LAURENCE DACADE 100MM MILA SEQUINED ANKLE BOOTS c08lP
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, [43] . The slightly lower binding, autophosphorylation and metabolic activities of M1 relative to those of human insulin are in agreement with the previous suggestion that the minor structural change caused by loss of the salt bridge between Asn A21 and Arg B22 leads to a (small) reduction in IR affinity [44] . In consequence, the slight reduction in metabolic activity of insulin glargine vs. human insulin derives predominantly from altered structural features at the carboxy-terminus of the B-chain and rather than from the amino acid substitution at A21.

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are metabolically active. Since a ratio of 50∶50 for parent compound and M1/M2 was reported in tissue samples derived from the site of injection in humans adidas Performance TERREX AGRAVIC GTX Hiking shoes basic green/shock yellow r8FTW1IBY
and in vitro metabolism of insulin glargine resulted into 46% to 98% appearance of M1 Faith JONNY Sandals black r1vyKyzd6K
, it is reasonable to assume that the metabolites of insulin glargine will contribute to the blood glucose lowering profile observed after administration of insulin glargine, dependent on the extent of their generation at the subcutaneous injection site and in plasma in the course of carboxypeptidase action [11] .

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